讲座：Mechanistic Studies of PHB Synthases
来源：
发布时间：2015-07-01 点击量：
报告题目：Mechanistic Studies of PHB Synthases
报告人：Ping Li (professor)
Kansas State University （USA）, Department of Chemistry
时间：2012年7月4日（周三）13：30
地点：行政楼一楼报告厅
报告摘要：
PHB synthases catalyze the conversion of 3-hydroxybutyryl coenzyme A (HBCoA) to PHB with a molecular mass of 1.5 MDa. The polymerization involves covalent catalysis with a Cys. Two mechanisms for elongation have been proposed. The first involves an active site composed of two monomers in which the growing hydroxybutyrate (HB) chain alternates between Cys on each monomer. The second involves covalently attached intermediates and noncovalent (HB)nCoA intermediates. Three strategies have been developed to distinguish between these models. Our results support the mechanism involving covalently and noncovalently bound intermediates. In addition, a loading site is proposed for the priming process. Understanding the mechanism of polymerization will facilitate us to engineer PHB synthases and to produce polymers in a controlled fashion.
报告人简介：
? 7/2011-present: Assistant Professor, Kansas State University, USA.
? 8/2006-4/2011:NIH postdoctoral fellow at Massachusetts Institute of Technology, USA. (Adviser: JoAnne Stubbe)
? 8/2000-7/2006: Postdoctoral fellow (12/2004-7/2006) and graduate research assistant (8/2000-11/2004) at Duke University, USA. (Adviser: Barbara Ramsay Shaw)
? 9/1995-5/1998: Graduate research assistant at Shanghai Institute of Organic Chemistry, China.
? 9/1993-5/1995: Undergraduate research assistant at Hangzhou University, China.
